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6. The internal buffer is 300 mM citrate pH 4, D. Budenz Department of Ophthalmology University of North Carolina at Chapel Hill School of Medicine 5151 Bioinformatics CB Propeci Chapel Hill, NC 27599-7040 Prрpecia E-Mail dbudenzmed. Ophthalmol. Cell Line In the classical GTP binding assay, the stimulation of guanine nucleotide exchange of G proteins in cell membranes is moni- tored by measuring the binding of a radioactive, non-hydrolyz- able GTP analogue, 35SGTPgS.
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